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human t cell leukemia cell line hpb  (DSMZ)


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    Structured Review

    DSMZ human t cell leukemia cell line hpb
    Human T Cell Leukemia Cell Line Hpb, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+t+cell+leukemia+cell+line+hpb/HPB-ALL/us12441796-1233-10-17
    Average 95 stars, based on 74 article reviews
    human t cell leukemia cell line hpb - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: Binding molecules for BCMA and CD3
    Article Snippet: Then, the cells are incubated for further 30 min with 30 μl of an anti-His Fab/Alexa488 solution (Micromet; 30 μg/ml).

    Binding Assay:

    Article Title: Bispecific antibody constructs for CDH3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human CDH3 and CD3 and to cyno CDH3 and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and cyno CDH3, respectively, the human CDH3 positive epidermoid carcinoma cell line A431, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line HSC-F. ..

    Article Title: Binding molecules for BCMA and CD3
    Article Snippet: Scatchard-Based Determination of Bispecific-Antibody Affinity to Human and Macaque BCMA For Scatchard analysis, saturation binding experiments are performed using a monovalent detection system developed at Micromet (anti-His Fab/Alexa 488) to precisely determine monovalent binding of the bispecific antibodies to the respective cell line. .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human and macaque BCMA and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and macaque BCMA, respectively, the human multiple myeloma cell line NCI-H929 expressing native human BCMA, CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) and the CD3-expressing macaque T cell line 4119LnPx (Knappe A, et al., Blood, 2000, 95, 3256-3261). .. Then, the cells are incubated for further 30 min with 30 μl of an anti-His Fab/Alexa488 solution (Micromet; 30 μg/ml).

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Flow Cytometry:

    Article Title: Bispecific antibody constructs for CDH3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human CDH3 and CD3 and to cyno CDH3 and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and cyno CDH3, respectively, the human CDH3 positive epidermoid carcinoma cell line A431, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line HSC-F. ..

    Article Title: Binding molecules for BCMA and CD3
    Article Snippet: Scatchard-Based Determination of Bispecific-Antibody Affinity to Human and Macaque BCMA For Scatchard analysis, saturation binding experiments are performed using a monovalent detection system developed at Micromet (anti-His Fab/Alexa 488) to precisely determine monovalent binding of the bispecific antibodies to the respective cell line. .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human and macaque BCMA and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and macaque BCMA, respectively, the human multiple myeloma cell line NCI-H929 expressing native human BCMA, CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) and the CD3-expressing macaque T cell line 4119LnPx (Knappe A, et al., Blood, 2000, 95, 3256-3261). .. Then, the cells are incubated for further 30 min with 30 μl of an anti-His Fab/Alexa488 solution (Micromet; 30 μg/ml).

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Transfection:

    Article Title: Bispecific antibody constructs for CDH3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human CDH3 and CD3 and to cyno CDH3 and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and cyno CDH3, respectively, the human CDH3 positive epidermoid carcinoma cell line A431, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line HSC-F. ..

    Article Title: Binding molecules for BCMA and CD3
    Article Snippet: Scatchard-Based Determination of Bispecific-Antibody Affinity to Human and Macaque BCMA For Scatchard analysis, saturation binding experiments are performed using a monovalent detection system developed at Micromet (anti-His Fab/Alexa 488) to precisely determine monovalent binding of the bispecific antibodies to the respective cell line. .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human and macaque BCMA and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and macaque BCMA, respectively, the human multiple myeloma cell line NCI-H929 expressing native human BCMA, CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) and the CD3-expressing macaque T cell line 4119LnPx (Knappe A, et al., Blood, 2000, 95, 3256-3261). .. Then, the cells are incubated for further 30 min with 30 μl of an anti-His Fab/Alexa488 solution (Micromet; 30 μg/ml).

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for CDH19 and CD3
    Article Snippet: .. L1.2 transfected with human CDH19, the human melanoma cell lines CHL-1 and A2058 expressing native human CDH19, CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) and the CD3-expressing macaque T cell line 4119LnPx (Knappe A, et al., Blood, 2000, 95, 3256-3261) were used as antigen positive cell lines. .. After Ficoll density centrifugation and extensive washes with Dulbecco's PBS (Gibco), remaining erythrocytes were removed from PBMC via incubation with erythrocyte lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 100 μM EDTA).

    Article Title: Antibody constructs for CDH19 and CD3
    Article Snippet: .. HEK293 transfected with human CDH19 (see example 14) and CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) were used as antigen positive cell lines. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Expressing:

    Article Title: Binding molecules for BCMA and CD3
    Article Snippet: Scatchard-Based Determination of Bispecific-Antibody Affinity to Human and Macaque BCMA For Scatchard analysis, saturation binding experiments are performed using a monovalent detection system developed at Micromet (anti-His Fab/Alexa 488) to precisely determine monovalent binding of the bispecific antibodies to the respective cell line. .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human and macaque BCMA and CD3, bispecific antibodies were tested by flow cytometry using CHO cells transfected with human and macaque BCMA, respectively, the human multiple myeloma cell line NCI-H929 expressing native human BCMA, CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) and the CD3-expressing macaque T cell line 4119LnPx (Knappe A, et al., Blood, 2000, 95, 3256-3261). .. Then, the cells are incubated for further 30 min with 30 μl of an anti-His Fab/Alexa488 solution (Micromet; 30 μg/ml).

    Article Title: Antibody constructs for CDH19 and CD3
    Article Snippet: .. L1.2 transfected with human CDH19, the human melanoma cell lines CHL-1 and A2058 expressing native human CDH19, CD3-expressing human T cell leukemia cell line HPB-ALL (DSMZ, Braunschweig, ACC483) and the CD3-expressing macaque T cell line 4119LnPx (Knappe A, et al., Blood, 2000, 95, 3256-3261) were used as antigen positive cell lines. .. After Ficoll density centrifugation and extensive washes with Dulbecco's PBS (Gibco), remaining erythrocytes were removed from PBMC via incubation with erythrocyte lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 100 μM EDTA).

    Construct:

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Incubation:

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Purification:

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Concentration Assay:

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: .. Bispecific Binding and Interspecies Cross-Reactivity For confirmation of binding to human DLL3 and CD3 and to cyno DLL3 and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for MSLN and CD3
    Article Snippet: For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. For confirmation of binding to human MSLN and CD3 and to cyno MSLN and CD3, bispecific antibody constructs of the invention were tested by flow cytometry using CHO cells transfected with human MSLN, with human MSLN isoform (NM_013404=SEQ ID NO:232 and AY743922=SEQ ID NO:233), and with macaque MSLN, respectively, the human MSLN positive human cell line OVCAR-8, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..

    Article Title: Antibody constructs for DLL3 and CD3
    Article Snippet: CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. .. CHO cells transfected with human DLL3, with an artificial human DLL3 isoform (characterized by the point mutations F172C and L218P), and with macaque DLL3, respectively, the human DLL3 positive human lung carcinoma cell line SHP-77, CD3-expressing human T cell leukemia cell line HPB-all (DSMZ, Braunschweig, ACC483), and the cynomolgus CD3-expressing T cell line LnPx 4119 For flow cytometry 200,000 cells of the respective cell lines were incubated for 60 min at 4° C. with 50 μl of purified bispecific antibody construct at a concentration of 5 μg/ml. ..



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